Image of a 3D lattice structure of a tissue implanted directly onto a soft...
Image of a 3D lattice structure of a tissue implanted directly onto a soft living tissue.
Source: Terasaki Institute

Bioprinting tissues directly within the body

Researchers take a step closer to 3D printing living tissues in patients as they develop a specially-formulated bio-ink designed for printing directly in the body.

In the TV series Westworld, human body parts are built on robotic frames using 3D printers. While still far from this scenario, 3D printers are being increasingly used in medicine. For example, 3D printing can be used to produce parts of the body such as orthopedic joints and prosthetics, as well as portions of bone, skin and blood vessels. However, the majority of these tissues are created in an apparatus outside of the body and surgically implanted. Such a procedure may involve making large surgical incisions, posing the added risk of infection and increased recovery time for the patient. And since there is a time lapse between when the tissue is created and when it is implanted in the patient, further complications may occur. To prevent these complications, a team of scientists have developed a technology to print tissues directly in the body.

There are two basic components needed to produce an engineered tissue: (1) a fluid-like “bio-ink” that consists of a framework material mixed with living cells, and (2) growth factors to help the cells grow and develop into regenerated tissue. When developing tissues for direct implantation into the body, there are other things to consider: the construction of tissue would have to be conducted at body temperature (37°C), the tissue needs to be attached effectively to soft, live organ tissue and any procedural steps should not be harmful to the patient. One such harmful step in current methods is the application of harmful UV light necessary to solidify the constructed tissue.

A collaboration among Ali Khademhosseini, Ph.D., Director and CEO of the Terasaki Institute, David J Hoelzle, Ph.D., from the Ohio State University Department of Mechanical and Aerospace Engineering and Amir Sheikhi, Ph.D. from the Pennsylvania State University Department of Chemical Engineering, has produced a specially-formulated bio-ink designed for printing directly in the body.

“This bio-ink formulation is 3D printable at physiological temperature, and can be crosslinked safely using visible light inside the body.” said first author Ali Asghari Adib, Ph.D. In order to build the tissue, they used robotic 3D printing, which uses robotic machinery affixed with a nozzle. Bio-ink may be dispensed through the nozzle, much like an icing tube squeezes out writing gel, only in a highly-precise, programmable manner.

The team also worked on methods to attach pieces of the tissue formed with this bio-ink onto soft surfaces. In experiments attempting to attach the tissue onto pieces of raw chicken strips and agarose, the team employed a unique interlock technique using the robotic 3D printer and their specially-formulated bio-ink. The nozzle tip was modified to be able to penetrate the soft surfaces and fill the punctured space with bio-ink as it withdrew; this created an anchor for the tissue construct. As the nozzle tip reached the surface, it dispensed an additional blob of bio-ink to “lock in” the anchor. “The interlocking mechanism enables stronger attachments of the scaffolds to the soft tissue substrate inside the patient body,” said Asghari Adib.

Such improvements in tissue engineering are instrumental in providing lower-risk, minimally-invasive laparoscopic options for procedures such as the repair of tissue or organ defects, engineering/implanting patches to enhance ovarian function, or creating bio-functional hernia repair meshes. Such options would be safer for the patient, save time and be more cost-effective. Further modifications in tissue engineering design and the adjustment of other conditions may increase the potential for customization, thus leading the way to limitless possibilities for enhancing patient health.

“Developing personalized tissues that can address various injuries and ailments is very important for the future of medicine. The work presented here addresses an important challenge in making these tissues, as it enables us to deliver the right cells and materials directly to the defect in the operating room,” said Khademhosseini, “This work synergizes with our Personalized Implant Technology Platform at the Terasaki Institute which aims to develop approaches that address the variability in tissue defects in patients.”

Subscribe to our newsletter

Related articles

3D biocomposites can repair large bone defects

3D biocomposites can repair large bone defects

Loosening hip implants can cause major damage to the bone and a simple replacement won’t suffice to carry the load during movements. Researchers have turned to bioprinting to solve this problem.

A 3D printed device to excite nerves

A 3D printed device to excite nerves

A tiny, thin-film electrode with a 3D-printed housing has been implanted in the peripheral nervous system of songbirds, where it successfully recorded electrical impulses that drive vocalizations.

3D printing cells to produce human tissue

3D printing cells to produce human tissue

Engineers have developed a method to 3D print cells to produce human tissue such as ligaments and tendons, a process that will greatly improve a patient's recovery.

3D printing enables tissue with customized shape

3D printing enables tissue with customized shape

3D-printed chambers with personalized shapes will be used to grow transplantable tissue that can take the shape of a wound to be closed.

3D printing congress: AM Medical Days 2021

3D printing congress: AM Medical Days 2021

The first edition of the new conference series AM Medical Days 2021 starts with the focus on "Medical AM: How to apply it to patients?".

Aerogel: the micro structural material of the future

Aerogel: the micro structural material of the future

Scientists from Empa were able to 3D print stable well-shaped microstructures made from silica aerogels for use in biotechnology and precision engineering.

3D printable biomaterial for personalised medicine

3D printable biomaterial for personalised medicine

Marc Knebel, head of Medical Systems at Evonik, explains the benefits and applications of the new high-performance polymer VESTAKEEP Care M40 3DF.

Implant shows promise for regenerating bone

Implant shows promise for regenerating bone

Researchers have developed a regenerative implant that could help repair bone-deep damage following physical trauma, surgery or osteoporosis.

Insulin-producing implant for diabetics

Insulin-producing implant for diabetics

Bioengineers are using 3D printing and smart biomaterials to create an insulin-producing implant for type 1 diabetes patients.

Popular articles

Subscribe to Newsletter